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sc 32233  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sc 32233
    Sc 32233, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 21678 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gapdh/GAPDH+Antibody/pmc13655259-68-17-18
    Average 97 stars, based on 21678 article reviews
    sc 32233 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Cell-Signaling:

    Article Title: Inhibition of Translesion DNA Synthesis Sensitizes BRCA-Deficient Ovarian Cancer to PARP Inhibitors
    Article Snippet: .. The following primary antibodies were used: anti-ubiquityl-PCNA (Lys164) (13439, Cell 180 Signaling Technology, 1:1000, RRID: AB_2798219), anti-PCNA (2586, Cell Signaling 181 Technology, 1:1000, RRID: AB_2160343), anti-Rad18 (8033, Cell Signaling Technology, 1:1000, 182 RRID: AB_10858879), anti-FANCM (38199, Cell Signaling Technology, 1:1000, RRID: 183 AB_3741495), anti-XPF (13465, Cell Signaling Technology, 1:2000, RRID: AB_2798227), anti-184 Histone H3 (4499S, Cell Signaling Technology, 1:3000, RRID: AB_10544537), anti-Polη (A301-185 231A, Bethyl Laboratories, 1:1000, RRID: AB_890600), anti-Polκ (A301-977A, Bethyl 186 Laboratories, 1:1000, RRID: AB_1548020), anti-Polι (A301-304A, Bethyl Laboratories, 1:1000, 187 RRID: AB_937818), anti-GAPDH (sc-365062, Santa Cruz Biotechnology, 1:5000, RRID: 188 AB_10847862), anti-REV1 (sc-393022, Santa Cruz Biotechnology, 1:1000, RRID: AB_2885169), 189 anti-p53 (MS-104-P1, ThermoFisher, 1:1000, RRID: AB_64408), anti-p21 (sc-6246, Santa Cruz 190 Biotechnology, 1:1000, RRID: AB_628073), anti-BRCA1 (MA1-23164, ThermoFisher, 1:1000, 191 RRID: AB_2067334), anti-pRPA32 (S4/S8) (A300-245A, Bethyl Laboratories, 1:1000, RRID: 192 AB_210547), anti-RPA32 (MABE285, Millipore Sigma, 1:2000, RRID: AB_11213221), anti-193 γH2AX (05-636, Millipore Sigma, 1:1000, RRID: AB_309864), anti-H2AX (7631, Cell Signaling 194 Technology, 1:1000, RRID: AB_10860771), anti-β-Actin (4970, Cell Signaling Technology, 195 1:1000, RRID: AB_2223172), and anti-Vinculin (sc-73614, Santa Cruz Biotechnology, 1:5000, 196 RRID: AB_1131294). ..

    Article Title: Aryl hydrocarbon receptor is critical for both AR-dependent and AR-indifferent enzalutamide resistance in castration-resistant prostate cancer.
    Article Snippet: The introduction of next-generation androgen receptor signaling inhibitors (ARSIs) like enzalutamide (ENZ), has improved the clinical management of castration-resistant prostate cancer (CRPC).. However, acquired resistance to these therapies often develops rapidly, and the underlying resistance mechanisms remain largely unclear.. Here, we identified the aryl hydrocarbon receptor (AHR) as a crucial operator of ENZ-resistant CRPC.

    Article Title: Boronate ester-crosslinked GelMA composite bioink for 3D bioprinting of cell-laden corneal stroma and in vivo evaluation in rabbits
    Article Snippet: Immunoreactive bands were visualized using enhanced chemiluminescence (ECL) reagents (GE Healthcare, United Kingdom). .. The primary antibodies used were anti-α-SMA (19245S, Cell Signaling Technology, USA) and anti-GAPDH (sc-47724, Santa Cruz Biotechnology, USA). .. The HRPconjugated secondary antibodies used were donkey antirabbit IgG (NA934V, Cytiva, United Kingdom) and sheep anti-mouse IgG (NA931V, Cytiva).

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: .. The following primary antibodies were used for Western blot analysis: anti-Zta MAb (Santa Cruz Biotechnology, Cat# sc-53904), anti-BMRF1 MAb (Santa Cruz Biotechnol ogy, Cat# SC-58121), anti-VCA/p18 (ThermoFisher, Cat# MAB8184), anti-GAPDH (Santa Cruz Biotechnology, Cat# SC-47724), anti-phospho-NF-κB p65 (Ser536) (Cell Signaling Technology, Cat# 3033T), anti-NF-κB p65 (Santa Cruz Biotechnology, Cat# SC-8008), anti-phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology, Cat# 4376T), antiERK1/2 (Santa Cruz Biotechnology, Cat# SC-514302), anti-phospho-p38 (Thr180/Tyr182) (Cell Signaling Technology, Cat# 4511T), and anti-p38 (Santa Cruz Biotechnology, Cat# SC-7972). ..

    Mass Spectrometry:

    Article Title: Inhibition of Translesion DNA Synthesis Sensitizes BRCA-Deficient Ovarian Cancer to PARP Inhibitors
    Article Snippet: .. The following primary antibodies were used: anti-ubiquityl-PCNA (Lys164) (13439, Cell 180 Signaling Technology, 1:1000, RRID: AB_2798219), anti-PCNA (2586, Cell Signaling 181 Technology, 1:1000, RRID: AB_2160343), anti-Rad18 (8033, Cell Signaling Technology, 1:1000, 182 RRID: AB_10858879), anti-FANCM (38199, Cell Signaling Technology, 1:1000, RRID: 183 AB_3741495), anti-XPF (13465, Cell Signaling Technology, 1:2000, RRID: AB_2798227), anti-184 Histone H3 (4499S, Cell Signaling Technology, 1:3000, RRID: AB_10544537), anti-Polη (A301-185 231A, Bethyl Laboratories, 1:1000, RRID: AB_890600), anti-Polκ (A301-977A, Bethyl 186 Laboratories, 1:1000, RRID: AB_1548020), anti-Polι (A301-304A, Bethyl Laboratories, 1:1000, 187 RRID: AB_937818), anti-GAPDH (sc-365062, Santa Cruz Biotechnology, 1:5000, RRID: 188 AB_10847862), anti-REV1 (sc-393022, Santa Cruz Biotechnology, 1:1000, RRID: AB_2885169), 189 anti-p53 (MS-104-P1, ThermoFisher, 1:1000, RRID: AB_64408), anti-p21 (sc-6246, Santa Cruz 190 Biotechnology, 1:1000, RRID: AB_628073), anti-BRCA1 (MA1-23164, ThermoFisher, 1:1000, 191 RRID: AB_2067334), anti-pRPA32 (S4/S8) (A300-245A, Bethyl Laboratories, 1:1000, RRID: 192 AB_210547), anti-RPA32 (MABE285, Millipore Sigma, 1:2000, RRID: AB_11213221), anti-193 γH2AX (05-636, Millipore Sigma, 1:1000, RRID: AB_309864), anti-H2AX (7631, Cell Signaling 194 Technology, 1:1000, RRID: AB_10860771), anti-β-Actin (4970, Cell Signaling Technology, 195 1:1000, RRID: AB_2223172), and anti-Vinculin (sc-73614, Santa Cruz Biotechnology, 1:5000, 196 RRID: AB_1131294). ..

    Incubation:

    Article Title: Aryl hydrocarbon receptor is critical for both AR-dependent and AR-indifferent enzalutamide resistance in castration-resistant prostate cancer.
    Article Snippet: The introduction of next-generation androgen receptor signaling inhibitors (ARSIs) like enzalutamide (ENZ), has improved the clinical management of castration-resistant prostate cancer (CRPC).. However, acquired resistance to these therapies often develops rapidly, and the underlying resistance mechanisms remain largely unclear.. Here, we identified the aryl hydrocarbon receptor (AHR) as a crucial operator of ENZ-resistant CRPC.

    Article Title: Inhibition of CDC42 in adenomyosis reduces the proliferation of endometrial stromal cells and the migration of epithelial cells
    Article Snippet: The protein was extracted using RIPA lysis buffer (Beyotime, Beijing, China), and the protein concentration was determined using a BCA (Beyotime, Beijing, China) kit. .. The protein was separated by SDS-PAGE and transferred to a PVDF membrane, which was blocked in 5% milk and incubated overnight with anti-CDC42 rabbit monoclonal antibody (1:500; Cat No. 10155-1-AP; Proteintech, China), anti-β-actin (1:1,000; Cat No. sc-47778; Santa Cruz, USA) and anti-GAPDH (1:1,000; Cat No. sc-47724; Santa Cruz, USA). .. After incubation with the secondary antibody of the corresponding species (1:3,000, Proteintech, China), an ECL kit (Absin, Beijing, China) was used to expose the protein substrate present on the membrane.

    Article Title: Programmable RNA targeting with clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas7-11 in zebrafish embryos and mammalian cells
    Article Snippet: Primary antibodies, i.e., Anti-FLAG M2 (Sigma), .. AR TIC LE IN PR ES S ARTICLE IN PRESS Anti-Tyrosinase (Novus), Anti-GAPDH (Santa Cruz) at 1:3,000 dilution, and Anti-beta Tubulin (Abcam) at 1:5,000 dilution, were diluted in blocking buffer and incubated with the membrane overnight at 4 °C with shaking. ..

    Membrane:

    Article Title: Aryl hydrocarbon receptor is critical for both AR-dependent and AR-indifferent enzalutamide resistance in castration-resistant prostate cancer.
    Article Snippet: The introduction of next-generation androgen receptor signaling inhibitors (ARSIs) like enzalutamide (ENZ), has improved the clinical management of castration-resistant prostate cancer (CRPC).. However, acquired resistance to these therapies often develops rapidly, and the underlying resistance mechanisms remain largely unclear.. Here, we identified the aryl hydrocarbon receptor (AHR) as a crucial operator of ENZ-resistant CRPC.

    Article Title: Inhibition of CDC42 in adenomyosis reduces the proliferation of endometrial stromal cells and the migration of epithelial cells
    Article Snippet: The protein was extracted using RIPA lysis buffer (Beyotime, Beijing, China), and the protein concentration was determined using a BCA (Beyotime, Beijing, China) kit. .. The protein was separated by SDS-PAGE and transferred to a PVDF membrane, which was blocked in 5% milk and incubated overnight with anti-CDC42 rabbit monoclonal antibody (1:500; Cat No. 10155-1-AP; Proteintech, China), anti-β-actin (1:1,000; Cat No. sc-47778; Santa Cruz, USA) and anti-GAPDH (1:1,000; Cat No. sc-47724; Santa Cruz, USA). .. After incubation with the secondary antibody of the corresponding species (1:3,000, Proteintech, China), an ECL kit (Absin, Beijing, China) was used to expose the protein substrate present on the membrane.

    Article Title: Programmable RNA targeting with clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas7-11 in zebrafish embryos and mammalian cells
    Article Snippet: Primary antibodies, i.e., Anti-FLAG M2 (Sigma), .. AR TIC LE IN PR ES S ARTICLE IN PRESS Anti-Tyrosinase (Novus), Anti-GAPDH (Santa Cruz) at 1:3,000 dilution, and Anti-beta Tubulin (Abcam) at 1:5,000 dilution, were diluted in blocking buffer and incubated with the membrane overnight at 4 °C with shaking. ..

    Western Blot:

    Article Title: Aryl hydrocarbon receptor is critical for both AR-dependent and AR-indifferent enzalutamide resistance in castration-resistant prostate cancer.
    Article Snippet: The introduction of next-generation androgen receptor signaling inhibitors (ARSIs) like enzalutamide (ENZ), has improved the clinical management of castration-resistant prostate cancer (CRPC).. However, acquired resistance to these therapies often develops rapidly, and the underlying resistance mechanisms remain largely unclear.. Here, we identified the aryl hydrocarbon receptor (AHR) as a crucial operator of ENZ-resistant CRPC.

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: .. The following primary antibodies were used for Western blot analysis: anti-Zta MAb (Santa Cruz Biotechnology, Cat# sc-53904), anti-BMRF1 MAb (Santa Cruz Biotechnol ogy, Cat# SC-58121), anti-VCA/p18 (ThermoFisher, Cat# MAB8184), anti-GAPDH (Santa Cruz Biotechnology, Cat# SC-47724), anti-phospho-NF-κB p65 (Ser536) (Cell Signaling Technology, Cat# 3033T), anti-NF-κB p65 (Santa Cruz Biotechnology, Cat# SC-8008), anti-phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology, Cat# 4376T), antiERK1/2 (Santa Cruz Biotechnology, Cat# SC-514302), anti-phospho-p38 (Thr180/Tyr182) (Cell Signaling Technology, Cat# 4511T), and anti-p38 (Santa Cruz Biotechnology, Cat# SC-7972). ..

    SDS Page:

    Article Title: Inhibition of CDC42 in adenomyosis reduces the proliferation of endometrial stromal cells and the migration of epithelial cells
    Article Snippet: The protein was extracted using RIPA lysis buffer (Beyotime, Beijing, China), and the protein concentration was determined using a BCA (Beyotime, Beijing, China) kit. .. The protein was separated by SDS-PAGE and transferred to a PVDF membrane, which was blocked in 5% milk and incubated overnight with anti-CDC42 rabbit monoclonal antibody (1:500; Cat No. 10155-1-AP; Proteintech, China), anti-β-actin (1:1,000; Cat No. sc-47778; Santa Cruz, USA) and anti-GAPDH (1:1,000; Cat No. sc-47724; Santa Cruz, USA). .. After incubation with the secondary antibody of the corresponding species (1:3,000, Proteintech, China), an ECL kit (Absin, Beijing, China) was used to expose the protein substrate present on the membrane.

    Blocking Assay:

    Article Title: Programmable RNA targeting with clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas7-11 in zebrafish embryos and mammalian cells
    Article Snippet: Primary antibodies, i.e., Anti-FLAG M2 (Sigma), .. AR TIC LE IN PR ES S ARTICLE IN PRESS Anti-Tyrosinase (Novus), Anti-GAPDH (Santa Cruz) at 1:3,000 dilution, and Anti-beta Tubulin (Abcam) at 1:5,000 dilution, were diluted in blocking buffer and incubated with the membrane overnight at 4 °C with shaking. ..

    Analysis:

    Article Title: Interleukin-17A directly triggers Epstein-Barr virus lytic reactivation in latency I human B-cell models.
    Article Snippet: .. The following primary antibodies were used for Western blot analysis: anti-Zta MAb (Santa Cruz Biotechnology, Cat# sc-53904), anti-BMRF1 MAb (Santa Cruz Biotechnol ogy, Cat# SC-58121), anti-VCA/p18 (ThermoFisher, Cat# MAB8184), anti-GAPDH (Santa Cruz Biotechnology, Cat# SC-47724), anti-phospho-NF-κB p65 (Ser536) (Cell Signaling Technology, Cat# 3033T), anti-NF-κB p65 (Santa Cruz Biotechnology, Cat# SC-8008), anti-phospho-ERK1/2 (Thr202/Tyr204) (Cell Signaling Technology, Cat# 4376T), antiERK1/2 (Santa Cruz Biotechnology, Cat# SC-514302), anti-phospho-p38 (Thr180/Tyr182) (Cell Signaling Technology, Cat# 4511T), and anti-p38 (Santa Cruz Biotechnology, Cat# SC-7972). ..



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